Blood tests for strength training and bodybuilding
For people who lift weights, bodybuilders and strength athletes, including anyone taking creatine.
11 tests explainedAI-verified against cited sources. Awaiting expert reviewLast reviewed 2026-10-19
Lifting changes several blood tests. Hard sessions release muscle enzymes such as CK, AST and ALT, and extra muscle or creatine can raise creatinine without any kidney problem. Testosterone or anabolic steroids, if used, show up in hematocrit, cholesterol and LH. Knowing this helps you and your clinician read results in context.
The tests that matter most
Most important first. Each one links to its full page, where the note for this group sits near the top.
Creatinine
Creatinine comes from muscle, so more muscle, creatine supplements and high-protein diets can raise it without any change in how well the kidneys filter. In healthy people, a creatine-related rise is unlikely to mean kidney damage.
Sources: KDIGO (2024) and Journal of the International Society of Sports Nutrition (2021)
- Men, 15+
- 0.74–1.35 mg/dL
- Women, 16+
- 0.59–1.04 mg/dL
eGFR (Creatinine-based)
Most eGFR results are worked out from creatinine, so a very muscular person can get an eGFR that looks lower than their true kidney function. Kidney guidelines list bodybuilding among the reasons a creatinine-based estimate can be off.
Source: KDIGO (2024)
- Usual adult range
- 60 and above mL/min/1.73 m²
Cystatin C
Cystatin C is a second kidney marker that doesn't depend on muscle. Kidney guidelines say an eGFR from cystatin C may suit people whose only reason to doubt their creatinine is extra muscle. The same guidelines note that steroids, including anabolic steroids, can affect cystatin C as well as creatinine.
Source: KDIGO (2024)
- 18–49
- 0.63–1.03 mg/L
- 50+
- 0.67–1.21 mg/L
Creatine Kinase (CK), Total
CK leaks from muscle after training, and resistance work, especially the lowering (eccentric) part of a lift, tends to raise it the most. A high CK after lifting is common, and some people rise far more than others after the same session.
- Men, 19–49
- 26–366 U/L
- Women, 19–49
- 20–239 U/L
Aspartate Aminotransferase (AST)
AST is found in muscle as well as liver. In one study, a single hour of weightlifting raised AST in men new to lifting, while GGT and bilirubin stayed normal.
- Men, 14+
- 8–48 U/L
- Women, 14+
- 8–43 U/L
Alanine Aminotransferase (ALT)
ALT is usually read as a liver test, but heavy workouts can raise it too. An Endocrine Society statement notes that liver enzymes raised by heavy workouts can be misread as a sign of liver problems.
Sources: British Journal of Clinical Pharmacology (2008) and Endocrine Reviews (2014)
- Men, 18+
- 0–44 U/L
- Women, 18+
- 0–32 U/L
Hematocrit
Hematocrit is the share of blood made of red cells. Testosterone and anabolic steroids raise hemoglobin and hematocrit in a dose-related way, and a high count is a common side effect.
Source: Endocrine Reviews (2014)
- Men, 13+
- 37.5–51 %
- Women, 13+
- 34–46.6 %
HDL Cholesterol
Anabolic steroids lower HDL cholesterol, especially steroids taken by mouth. A low HDL is one of the lab signs the Endocrine Society says might suggest steroid use.
Source: Endocrine Reviews (2014)
- Usual adult range
- 40 and above mg/dL
LDL Cholesterol
Anabolic steroids tend to raise LDL cholesterol while lowering HDL. That pattern is linked to artery disease over time.
Source: Endocrine Reviews (2014)
- Usual adult range
- up to 99 mg/dL
Testosterone, Total
Taking testosterone or other steroids switches off the body's own testosterone signal. After stopping, natural testosterone can stay low for months or longer.
Source: Endocrine Reviews (2014)
- Men, 20+
- 264–916 ng/dL
- Men, 18–19
- 150–785 ng/dL
Luteinizing Hormone (LH)
LH is the brain's signal to the testes to make testosterone. A high testosterone with a suppressed LH and FSH is the pattern seen when testosterone comes from outside the body.
Source: Endocrine Reviews (2014)
- Usual adult range
- 1.7–8.6 mIU/mL
Ranges shown are the usual adult ranges labs print. The range on your own report is the one your result is judged against.
Getting results you can compare
- In one study, a single weightlifting session raised AST, ALT and CK for at least 7 days in men not used to lifting. The authors suggest a week without weightlifting before the start of drug trials.
- After lifting, CK can peak anywhere from a day to several days later and usually settles within about 7 to 9 days. Telling your clinician when you last trained hard helps them read CK, AST and ALT.
- If you take creatine or eat a very high-protein diet, mention it when you have kidney tests, since both can raise creatinine.
Sources: KDIGO (2024) and Journal of the International Society of Sports Nutrition (2021)
Good to know
- Anabolic steroids, including high-dose testosterone, raise hemoglobin and hematocrit, lower HDL, raise LDL and suppress LH and FSH, the signals that tell the body to make its own testosterone. These changes show up on routine tests, so they are worth knowing about when reading results.
Source: Endocrine Reviews (2014)
- Kidney guidelines list bodybuilding, high-protein diets, creatine supplements and anabolic steroids among things that change creatinine without changing kidney filtering. That is why a single creatinine-based result can mislead in this group.
Source: KDIGO (2024)
Questions you could ask
- Could my muscle mass or creatine use be affecting my creatinine and eGFR?
- Would a cystatin C test add anything for me?
- How long after a hard session should my next blood draw be?
- How often should we recheck my hematocrit and cholesterol?
Sources
- KDIGO 2024 Clinical Practice Guideline for the Evaluation and Management of Chronic Kidney Disease, KDIGO (2024)
- Common questions and misconceptions about creatine supplementation: what does the scientific evidence really show?, Journal of the International Society of Sports Nutrition (2021)
- Muscular exercise can cause highly pathological liver function tests in healthy men, British Journal of Clinical Pharmacology (2008)
- Adverse health consequences of performance-enhancing drugs: an Endocrine Society scientific statement, Endocrine Reviews (2014)
- Creatine-kinase- and exercise-related muscle damage implications for muscle performance and recovery, Journal of Nutrition and Metabolism (2012)
AI-verified against cited sources. Awaiting expert review. Last reviewed 2026-10-19. How we build and check these pages.
Educational information, not medical advice. Blood Test Tracker doesn’t diagnose or treat, and it doesn’t replace your clinician. Talk with your clinician before changing medications, supplements or care.